rabbit anti rat c3  (Novus Biologicals)

Journal: bioRxiv

Article Title: Complement inhibition by C3-siRNA treatment prevents AChR loss and reduces complement activation in the rat Passive Transfer Myasthenia Gravis (PTMG)

doi: 10.1101/2025.08.31.673367

Figure Lengend Snippet:

Article Snippet: After washing 3 times with 300 μl/well washing buffer (PBS, 0.2% Tween), 25 μl/well of capture rabbit anti-rat C3 antibody (1 μg/ml, Hycult #HP8022-100UG) were added and incubated for 1-2 hours at RT with shaking or overnight at 4°C.

Techniques: Injection, Clinical Proteomics

Journal: bioRxiv

Article Title: Complement inhibition by C3-siRNA treatment prevents AChR loss and reduces complement activation in the rat Passive Transfer Myasthenia Gravis (PTMG)

doi: 10.1101/2025.08.31.673367

Figure Lengend Snippet: A) After baseline measurements on day 0, subcutaneous treatment injections were administered on day 1 and 8. Intermediate testing and blood collection were performed on day 4, 7 and 11 (not shown). On day 15, indicated with a red arrow, PTMG was induced using mAb35, while non-MG animals received a saline injection of the same volume. B-C) Plasma C3 expression was reduced by the sc administration of C3-siRNA at various dose levels in B) non-MG animals and C) PTMG animals. For animals receiving 2 x 30 mg/kg treatment, doses were administered on days 1 and 8. For animals receiving 1 x 10 or 1 x 30 mg/kg doses, administration occurred on day 8, as indicated by the black arrows on the graphs. Saline-treated animals showed no significant change in C3 levels over time. Group averages are shown. D) Plasma C3 level changes in individual treatment groups on day 17, normalized to baseline levels on day 0. Individual plasma C3 levels per animal are shown. Statistical analysis was performed with Two-Way ANOVA (B-D), followed by One-Way ANOVA and post-hoc Bonferroni’s multiple comparison test (D); α=0.05; ****p<0.0001. A) Created in BioRender. Schöttler, A. (2025) https://BioRender.com/b4xxodd .

Article Snippet: After washing 3 times with 300 μl/well washing buffer (PBS, 0.2% Tween), 25 μl/well of capture rabbit anti-rat C3 antibody (1 μg/ml, Hycult #HP8022-100UG) were added and incubated for 1-2 hours at RT with shaking or overnight at 4°C.

Techniques: Saline, Injection, Clinical Proteomics, Expressing, Comparison

Journal: bioRxiv

Article Title: Complement inhibition by C3-siRNA treatment prevents AChR loss and reduces complement activation in the rat Passive Transfer Myasthenia Gravis (PTMG)

doi: 10.1101/2025.08.31.673367

Figure Lengend Snippet: C3 protein levels in plasma of healthy animals receiving a single treatment injection to establish the dose range of C3-siRNA for the PTMG study. After baseline measurements on day 0, C3-siRNA was administered on day 1 (black arrow) and plasma C3 protein levels analyzed on days 3, 8, 15, 22, and 29. Per group N=3. Group averages are shown.

Article Snippet: After washing 3 times with 300 μl/well washing buffer (PBS, 0.2% Tween), 25 μl/well of capture rabbit anti-rat C3 antibody (1 μg/ml, Hycult #HP8022-100UG) were added and incubated for 1-2 hours at RT with shaking or overnight at 4°C.

Techniques: Clinical Proteomics, Injection

Journal: bioRxiv

Article Title: Complement inhibition by C3-siRNA treatment prevents AChR loss and reduces complement activation in the rat Passive Transfer Myasthenia Gravis (PTMG)

doi: 10.1101/2025.08.31.673367

Figure Lengend Snippet: C3 protein levels in plasma of healthy animals receiving three consecutive treatment injections, with a cumulative dose equal to a single injection as shown in . After baseline measurements on day 0, C3-siRNA was administered on day 1, 2, and 3 (black arrows) and plasma C3 protein levels analyzed on day 3, 8, 15, 22 and 29. Per group N=3. Group averages are shown.

Article Snippet: After washing 3 times with 300 μl/well washing buffer (PBS, 0.2% Tween), 25 μl/well of capture rabbit anti-rat C3 antibody (1 μg/ml, Hycult #HP8022-100UG) were added and incubated for 1-2 hours at RT with shaking or overnight at 4°C.

Techniques: Clinical Proteomics, Injection

Journal: bioRxiv

Article Title: Complement inhibition by C3-siRNA treatment prevents AChR loss and reduces complement activation in the rat Passive Transfer Myasthenia Gravis (PTMG)

doi: 10.1101/2025.08.31.673367

Figure Lengend Snippet: Three days after treatment injection, hepatic C3 mRNA expression was significantly repressed in all C3-siRNA treated animals (left). 30 days after C3-siRNA injection, the expression was still reduced to 50% and ca. 25% in animals that received 10 or 30 mg/kg C3-siRNA (single and cumulative) compared to control animals. Each dot indicates one animal. Statistical analysis as performed using One-Way ANOVA with post-hoc Bonferroni’s multiple comparison test; α=0.05; **p=0.003, ***p<0.0004, ****p<0.0001.

Article Snippet: After washing 3 times with 300 μl/well washing buffer (PBS, 0.2% Tween), 25 μl/well of capture rabbit anti-rat C3 antibody (1 μg/ml, Hycult #HP8022-100UG) were added and incubated for 1-2 hours at RT with shaking or overnight at 4°C.

Techniques: Injection, Expressing, Control, Comparison

Journal: bioRxiv

Article Title: Complement inhibition by C3-siRNA treatment prevents AChR loss and reduces complement activation in the rat Passive Transfer Myasthenia Gravis (PTMG)

doi: 10.1101/2025.08.31.673367

Figure Lengend Snippet: One PTMG animal treated with 30 mg/kg C3-siRNA showed no siRNA in the liver. Assuming a misinjection of C3-siRNA, this animal was excluded from the analysis. Each dot indicates one animal. Statistical analysis was performed using One-Way ANOVA with post-hoc Bonferroni’s multiple comparison test; α=0.05; ***p=0.0009, ****p<0.0001.

Article Snippet: After washing 3 times with 300 μl/well washing buffer (PBS, 0.2% Tween), 25 μl/well of capture rabbit anti-rat C3 antibody (1 μg/ml, Hycult #HP8022-100UG) were added and incubated for 1-2 hours at RT with shaking or overnight at 4°C.

Techniques: Comparison

Journal: bioRxiv

Article Title: Complement inhibition by C3-siRNA treatment prevents AChR loss and reduces complement activation in the rat Passive Transfer Myasthenia Gravis (PTMG)

doi: 10.1101/2025.08.31.673367

Figure Lengend Snippet: A) Grip strength decreased in PTMG animals after disease induction (red arrow) with a dose-dependent effect of the C3-siRNA treatment. B) Grip strength on day 17 with non-MG animals pooled into one group. C) Disease scoring increased in PTMG animals after disease induction (red arrow), with an evident dose dependent effect of the C3-siRNA treatment. D) Disease score on day 17 with non-MG animals pooled into one group. In A) and C) group averages are shown. In B) and D) each dot represents one individual animal; horizontal bars indicate group means. Statistical analysis was performed with Two-Way ANOVA (A-C), followed by One-Way ANOVA and post-hoc Bonferroni’s multiple comparison test (B) or Kruskal-Wallis test (D); α=0.05; *p=0.0408, **p=0.0021, ****p<0.0001.

Article Snippet: After washing 3 times with 300 μl/well washing buffer (PBS, 0.2% Tween), 25 μl/well of capture rabbit anti-rat C3 antibody (1 μg/ml, Hycult #HP8022-100UG) were added and incubated for 1-2 hours at RT with shaking or overnight at 4°C.

Techniques: Comparison

Journal: PLoS ONE

Article Title: Systemic Administration of Abeta mAb Reduces Retinal Deposition of Abeta and Activated Complement C3 in Age-Related Macular Degeneration Mouse Model

doi: 10.1371/journal.pone.0065518

Figure Lengend Snippet: Primary antibodies used for IHC detection in cfh−/− mouse retinae.

Article Snippet: Although the same primary antibody was used for the detection of Aβ deposition as in the remainder of the study, (4G8, ), this was biotin-labelled and detected with Alexa Fluor 488 streptavidin, and a different primary antibody was used to detect total C3, (Hycult biotech, cat# HP8022) a rabbit polyclonal anti-Rat C3 antibody which gave low background for IHC staining, (data from this study is described solely in the final part of the Results section and was the only occasion that the antibody combination described above was used in this work).

Techniques:

Journal: PLoS ONE

Article Title: Systemic Administration of Abeta mAb Reduces Retinal Deposition of Abeta and Activated Complement C3 in Age-Related Macular Degeneration Mouse Model

doi: 10.1371/journal.pone.0065518

Figure Lengend Snippet: Log scored means +/− SE for Amyloid β (4G8+, blue) and complement C3 (C3+, red, detected with rabbit anti-rat polyclonal Ab to total C3, Hycult, HP80222, ), deposition in retinal sections after 3 months of dosing with: (i) 6F6, [solid bars]; (ii) IgG, IgG2A isotype specific negative control Ab, [open bars], at 600 µg weekly doses. Group sizes, n = numbers of eyes treated, are marked at the base of the bars in parentheses. Differences of **p = 0.0232, for Amyloid β deposition and of *p = 0.0414 for C3 deposition were noted comparing 6F6 treated with IgG2A control. See SI .

Article Snippet: Although the same primary antibody was used for the detection of Aβ deposition as in the remainder of the study, (4G8, ), this was biotin-labelled and detected with Alexa Fluor 488 streptavidin, and a different primary antibody was used to detect total C3, (Hycult biotech, cat# HP8022) a rabbit polyclonal anti-Rat C3 antibody which gave low background for IHC staining, (data from this study is described solely in the final part of the Results section and was the only occasion that the antibody combination described above was used in this work).

Techniques: Negative Control

Journal: PLoS ONE

Article Title: Systemic Administration of Abeta mAb Reduces Retinal Deposition of Abeta and Activated Complement C3 in Age-Related Macular Degeneration Mouse Model

doi: 10.1371/journal.pone.0065518

Figure Lengend Snippet: Primary antibodies used for IHC detection in cfh−/− mouse retinae.

Article Snippet: Rabbit anti-rat polyclonal Ab to total C3 , Hycult Biotechnology , HP8022 , 1∶50.

Techniques:

Journal: PLoS ONE

Article Title: Systemic Administration of Abeta mAb Reduces Retinal Deposition of Abeta and Activated Complement C3 in Age-Related Macular Degeneration Mouse Model

doi: 10.1371/journal.pone.0065518

Figure Lengend Snippet: Log scored means +/− SE for Amyloid β (4G8+, blue) and complement C3 (C3+, red, detected with rabbit anti-rat polyclonal Ab to total C3, Hycult, HP80222, ), deposition in retinal sections after 3 months of dosing with: (i) 6F6, [solid bars]; (ii) IgG, IgG2A isotype specific negative control Ab, [open bars], at 600 µg weekly doses. Group sizes, n = numbers of eyes treated, are marked at the base of the bars in parentheses. Differences of **p = 0.0232, for Amyloid β deposition and of *p = 0.0414 for C3 deposition were noted comparing 6F6 treated with IgG2A control. See SI .

Article Snippet: Rabbit anti-rat polyclonal Ab to total C3 , Hycult Biotechnology , HP8022 , 1∶50.

Techniques: Negative Control